一、基于CRISPR的快速检测技术和平台：开发基于CRISPR的快速、高效、灵敏和精确的多功能现场检测和诊断的方法。

二、肿瘤电场治疗研究：在分子、细胞、组织和动物水平，研究不同种类肿瘤对交流可变电场（AEFields）治疗和联合治疗条件下的应答效率及机制。

三、肠道微生态在消化道疾病中的功能：运用基于CRISPRn/i（敲除、抑制）高通量体内外筛选平台，(1) 鉴定致病菌作用于宿主细胞的靶点；(2) 解析宿主细胞应答致病菌的分子机制；(3) 探索肠道微生态、肠道免疫、肠道神经和肠细胞的相互作用机制。

四、肿瘤免疫：运用基于CRISPRn/i（敲除、抑制）高通量体内外筛选平台，结合小鼠体内成瘤模型研究：(1) 肿瘤细胞向淋巴结转移的分子机制; (2) 肿瘤细胞逃逸免疫系统的分子机制; (3) 免疫细胞在肿瘤微环境浸润、激活（失活）的分子机制。

1998.09 ~ 2002.06     南京大学生物化学系                           理学学士；

2002.09 ~ 2009.04     中国科学院上海生化细胞所                 理学博士；

2005.03 ~ 2005.12     德国马克思普朗克生物化学研究所       访问博士；

2006.01 ~ 2006.04     丹麦哥本哈根分子病理研究所              访问博士；

2009.04 ~ 2010.03         中国科学院上海生化细胞所                            研究助理

2010.04 ~ 2015.04         美国加州大学旧金山分校细胞和组织学系       博士后

2015.05 ~ 2018.09         美国加州大学旧金山分校细胞和组织学系       associate specialist

2014.10 ~ 2015.03         美国斯坦福大学生物工程学系                         访问学者

2015.05 ~ 2017.04         美国格拉德斯通研究所                                   访问学者

1. Xiao Wang#, Xiaobao Deng#, Yidun Zhang#, Weiyi Dong, Qiao Rao, Qingmei Huang, Fei Tang, Rong Shen, Hongzhi Xu, Zhen Jin, Youzhi Tang* and Dan Du*. A rapid and sensitive one-pot platform Integrating fluorogenic RNA aptamers and CRISPR-Cas13a for visual detection of monkeypox virus. Biosens Bioelectron., 257 (2024) 116268, doi:10.1016/.bios 2024.116268. (*: co-corresponding author);

2. Xiao Wang#, Qiao Rao#, Zhengrong Lu#, Xiaobao Deng, Rong Shen, Rui Wang, Weiyi Dong, Xianhua Qi, Zhen Jin, Youzhi Tang*, Dan Du*. Rapid and sensitive Cas13a/Cas12a-based one-pot dual-target strategy to detect monkeypox virus and its co-infected viruses. Sci Bull., 2023, doi:10.1016/j.scib.2023.11.023 (*: co-corresponding author);

3. Xiao Wang, Rui Wang, Zhao-Sheng Zhang 3, Guang-Yu Zhang, Zhen Jin, Rong Shen, Dan Du*, You-Zhi Tang*. Semisynthetic pleuromutilin antimicrobials with therapeutic potential against methicillin-resistant Staphylococcus aureus by targeting 50S ribosomal subunit. Eur J Med Chem. 2022 Jul 5th;237:114341. doi: 10.1016/j.ejmech. 2022.114341 (*: co-corresponding author).

4. Xiao Wang, Ling-Ling Xu, Xiang-Yi Zuo, Jia-Wen Lin, Zhen Jin 1, Rong Shen,  Dan Du*, You-Zhi Tang*. Rapid detection of the New Delhi metallo-β-lactamase (NDM) gene by recombinase polymerase amplification. Infect Genet Evol. 2021 Jan;87:104678. doi: 10.1016/j.meegid.2020.104678(*: co-corresponding author). 

5. Wei Wang, Ru-Feng Yue, Zhen Jin, Li-Min He, Rong Shen, Dan Du*, You-Zhi Tang*. Efficiency comparison of apigenin-7-O-glucoside and trolox in antioxidative stress and anti-inflammatory properties. J Pharm Pharmacol. 2020 Nov;72(11):1645-1656. doi: 10.1111/jphp.13347(*: co-corresponding author). 

6. Dan Du*, Yoko Katsuno, Dominique Meyer, Erine H. Budi, Si-Han Chen, Hongjun Wang, Hartmut Koeppen, Rosemary Akhurst, Rik Derynck*. Smad3‐mediated recruitment of the methyltransferase SETDB1/ESET controls Snail1 expression and epithelial–mesenchymal transition. EMBO Reports., 2017 Dec 12th; 19(1), 135-155 (*: co-corresponding author). doi: 10.15252/embr.201744250 

7. Dan Du#, Assen, Roguev#, David E Gordon，Meng Chen, Si-Han Chen, John Paul Shen, Prashant Mali, Trey Ideker, Stanley Qi, Nevan Krogan. Genetic interaction mapping in mammalian cells using CRISPR interference. Nat. Methods., 2017 May 8th; 14(6), 577–580 (#: co-first author). doi:10.1038/nmeth.4286

8. Dan Du, Stanley Qi. CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Cold Spring Harb Protoc. 2016 Jan 4th; (1), pdb. prot090175. doi:10.1101/pdb.prot090 175.

9. Dan Du, Stanley Qi. An Introduction to CRISPR Technology for Genome Activation and Repression in Mammalian Cells. Cold Spring Harb Protoc. 2016 Jan 4th ; (1), pdb. top086835. doi:10.1101/pdb.top086835.

10. Dan Du, Feilai Xu, Lihou Yu, Chenyi Zhang, Xuefeng Lu, Haixin Yuan, Qin Huang, Fan Zhang, Xunwei Wu, Zhe Zhang, Xueliang Zhu, Xiaohui Zhang, Zhengjun Chen. Tight junction protein occludin regulates directional migration of epithelial cells. Dev Cell., 2010 Jan 19th, 18 (1), 52-63. doi: https://doi.org/10.1016/j.devcel.2009.12.008.

1. 科技部重大研发计划（微生物机制研究及微生物宿主互作靶标的筛选，2022YFA1304002，2022-2027，440万元）

2. 国家自然科学基金面上项目（81972823，2020-2023，60万元） 

3. 国家自然科学基金面上项目（82372788，2024-2027，73万元）

4. 中组部第16批海外高层次青年人才经费（ 200万元）

5. 闽江学者启动经费（200万元）

6. Human Frontier Science Program (HFSP) fellowship (as PI), “Role of the methyltransferase ESET in TGF-beta signaling and EMT”, 2011-2014. This award is around $160,980.

7. The Leukemia & Lymphoma Society FELLOW Award (as PI), “Functional crosstalk between SMYD3 and TGF-beta signaling to control cellular senescence”, 2011-2014. This award is around $165,000.