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代表性文章或专著：
一、通讯（共同通讯）作者文章（*）：
[1]. Y. Wang, J. Ye, X. Liu, Z. Zhang, F. Shang, X. Qi, Y. Zhang, J. Du, H. Sun, J. Xu, H. Chen*, M. Yu*, S. Le*, Mechanically weak and highly dynamic state of mechanosensitive titin Ig domains induced by proline isomerization, Nature Communications 16(1):2771(2025)
[2]. Y. Zhang, Z. Zhang, H. Sun, Z. Xue, Y. Wang, Z. Guo, S. Le*, & H. Chen*, Anomalous Force-Dependent Transition Rates Unveil Dual Pathways in Folding and Unfolding Dynamics of Acyl-coenzyme A Binding Protein, The Journal of Physical Chemistry Letters 16, 2479-2486 (2025)
[3]. Y. Zhang#, J. Du#, X. Liu, F. Shang, Y. Deng, J. Ye, Y. Wang, J.Yan, H. Chen*, M. Yu* & S. Le*, Multi-domain interaction mediated strength-building in human α-actinin dimers unveiled by direct single-molecule quantification, Nature Communications 15: 6151 (2024)
[4]. J. Xu, H. Sun, Z. Zhang, Z. Guo, S. Le*, H. Chen* , Folding and Misfolding Dynamics of Irisin Protein Revealed by Single-Molecule Magnetic Tweezers , The Journal of Physical Chemistry Letters 15, 11954-11960 (2024)
[5]. Z Xue, H Sun, H Hong, Z Zhang, Y Zhang, Z Guo, S. Le*, H Chen*, Comparative analysis of folding and unfolding dynamics and free-energy landscapes in homologous cold shock proteins with variable thermal stabilities, Physical Review Research 6 (2), 023170 (2024)
[6]. M. Yu, J. Lu, S. Le*, and J. Yan*, Unexpected Low Mechanical Stability of Titin I27 Domain at Physiologically Relevant Temperature, The Journal of Physical Chemistry Letters 12(33), 7914-7920 (2021)
[7]. M. Yu, Z. Zhao, Z. Chen, S. Le*, and J. Yan*, Modulating Mechanical Stability of Heterodimerization Between Engineered Orthogonal Helical Domains, Nature Communications 11: 4476 (2020)二、第一（共同第一）作者文章（#）：
[1]. S. Le#, M. Yu#, C. Fu#, JA Heier, S Martin, J. Hardin*, J. Yan* , Single-molecule force spectroscopy reveals intra- and intermolecular interactions of Caenorhabditis elegans HMP-1 during mechanotransduction, Proceedings of the National Academy of Sciences 121 (37), e2400654121 (2024)
[2]. M. Yu#, S. Le#, S. Barnett#, Z. Guo, X. Zhong, P. Kanchanawong*, and J. Yan*, Implementing Optogenetic Modulation in Mechanotransduction, Physical Review X 10, 021001 (2020)
[3]. S. Le#, M. Yu#, and J. Yan*, Direct Single-molecule Quantification Reveals Surprisingly High Mechanical Stability of Vinculin--Talin/alpha-Catenin Linkages, Science Advances 5 (12), eaav2720 (2019)
[4]. S. Le#, M. Yu#, and J. Yan*, Phosphorylation Reduces the Mechanical Stability of alpha-catenin/beta-catenin Complex, Angewandte Chemie International Edition 58(51) 18663-18669 (2019)
[5]. M. Yu#, S. Le#, Y. Ammon#, B. Goult, A. Akhmanova*, and J. Yan*, Force-dependent Regulation of KANK1-talin Complex at Focal Adhesions, Nano Letters 19 (9), 5982-5990 (2019)
[6]. S. Le, M. Yu, L. Hovan, Z. Zhao, J. Ervasti, and J. Yan*, Dystrophin as a Molecular Shock Absorber, ACS Nano 12(12):12140-12148 (2018)
[7]. M. Yu#, S. Le#, A. Efremov, X. Zeng, A. Bershadsky, and J. Yan*, Effects of Mechanical Stimuli on Profilin- and Formin-mediated Actin Polymerization, Nano Letters 18 (8), 5239-5247. (2018)
[8]. S. Le, X. Hu, M. Yao, H. Chen, M. Yu, X. Xu, F. Margadant, M. Sheetz*, and J. Yan*, Mechanotransmission and Mechanosensing of Human alpha-actinin 1, Cell Reports 21, 1-10. (2017)
[9]. S. Le#, E. Serrano#, R. Kawamura B. Carrasco, J. Yan*, and J.Alonso*, Bacillus Subtilis DprA and SsbA Promote the Assembly of a RecA Nucleoprotein Filament that Can Overcome RecX Inhibition during Natural Chromosomal Transformation, Nucleic Acids Research 45(15): 8873-8885 (2017)
[10]. S. Le#, M. Yao#, J. Chen, A. K. Efremov, S. Azimi, and J. Yan*, Disturbance-free Rapid Solution Exchange for Magnetic Tweezers Single-molecule Studies, Nucleic Acids Research 43(17): e113 (2015)
[11]. S. Le, H. Chen, X. Zhang, J. Chen, K. Patil, K. Muniyappa*, and J. Yan*, Mechanical Force Antagonizes the Inhibitory Effects of RecX on RecA Filaments Formation in M. tuberculosis, Nucleic Acids Research 42 (19): 11992-11999. (2014)
[12]. H. Fu#, S. Le#, H. Chen, K. Muniyappa*, and J. Yan*, Force and ATP Hydrolysis Dependent Regulation of RecA Nucleoprotein Filament by Single-stranded DNA Binding Protein, Nucleic Acids Research 41, 924 (2013)三、综述文章：
[1]. S. Le, M. Yu, A. Bershadsky*, and J.Yan*, Mechanical regulation of formin-dependent actin polymerization. Seminars In Cell & Developmental Biology 102: 73-80 (2020).
[2]. S. Le, M. Yu, and J. Yan*, Mechanical Regulation of Tension-transmission Supramolecular Linkages, Current Opinion in Solid State and Materials Science 25:100895 (2021).
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力学生物学（Mechanobiology）是一门融合生物学、物理学、医学、工程学与材料科学的新兴前沿交叉学科。近年来的研究表明，细胞黏附、迁移与分化，组织生长、修复与维持，胚胎发育，肿瘤生长与转移，免疫激活等一系列关键生理病理过程，均受到力学信号的调控。力学生物学旨在阐明力学信号在分子、亚细胞、细胞、组织、器官等不同层次对生命体行为进行调控的机制，并基于这些机制理解推动相关生物医学应用。
力学生物学的物质基础是一系列力敏感生物大分子及其通过动态相互作用形成的超分子复合物。这些力敏感分子与复合物通常动态富集于细胞的关键连接部位，如细胞间连接（包括胞间连接与紧密连接等）、细胞与胞外基质间的连接（如黏附连接等）、细胞骨架内部及骨架之间的连接，以及细胞骨架与细胞核等细胞器之间的连接。这些力敏感大分子及其复合物在特定时空的构象变化与相互作用的力学—化学耦合动态，构成了生命体在亚细胞、细胞、组织与器官等层次的力学功能涌现的分子基础。
本课题组聚焦生命医学问题，以“生物大分子构象与相互作用的力学—化学耦合动态调控机制”为核心研究方向，积极探索其在生物医学中的潜在应用。研究注重物理与生物、医学的交叉融合，综合运用动态单分子与单细胞力学操控及成像技术，并结合分子动力学模拟、高分子物理建模等手段，从而在实验与理论上协同理解生物大分子构象与相互作用的力学—化学耦合动态调控，进而逐步揭示在生理与病理过程中发挥关键作用的蛋白质、核酸等生物大分子功能实现的物理机制。
课题组当前之课题方向简述：
1). 细胞黏附、胞间连接关键力敏感蛋白质，及其介导的力敏感信号通路力学-化学-生物耦合调控机制的单分子与单细胞研究；
2). 细胞骨架与细胞核骨架关键力敏感蛋白质，及其介导的力敏感信号通路力学-化学-生物耦合调控机制的单分子与单细胞研究；
3). 肌联蛋白（Titin）的构象（去折叠、复折叠、错误折叠、中间态等）、修饰（氧化、异构、磷酸化等）、互作（信号蛋白、分子伴侣等）的力学-化学-生物耦合调控机制研究；
4). 活细胞内力学、化学、生物信号的原位探测技术开发与应用；
5). 干预、调控蛋白质分子的AI辅助从头设计、优化与应用；